Using a powerful single-molecule imaging method they developed, a research team from the Broad Institute of MIT and Harvard has unveiled a dynamic view of how some cancer-related proteins interact in living cells. The technique relies on highly stable nanoparticle probes that brightly illuminate individual molecules for long periods of time. The researchers used their method to observe, for the first time, individual receptors as they move around the cell membrane, attaching to and then letting go of other receptors to alter signaling within the cell. Described in the journal Cell, the work demonstrates the method's potential for investigating other receptors and molecules, and for improved drug screening to better understand the effects of therapeutics on living cells. 'With our photostable probes, we can map out the entire lifespan of these molecules in their native environment and see things that have never been observable before,' says study leader Sam Peng, a Broad Institute...
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